Analysing RNA-Seq data with the DESeq package
نویسنده
چکیده
Abstract A basic task in the analysis of count data from RNA-Seq is the detection of differentially expressed genes. The count data are presented as a table which reports, for each sample, the number of reads that have been assigned to a gene. Analogous analyses also arise for other assay types, such as comparative ChIP-Seq. The package DESeq provides a method to test for differential expression by use of a shrinkage estimtor for the variance. This vignette explains the use of the package. For an exposition of the statistical method employed, please see our paper [1].
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